FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba121.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba121.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40070777
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG27700.09.96443248
ACGTCCG26950.09.85712932
CGGGTAT28500.09.8049181
GTCGGTT105700.09.3672211
GTCCGTT52050.08.9478131
GTCCGGT53600.08.8821511
CTCGTAT57750.08.6620331
GTCCATT215450.08.4865891
GACGTAT24100.08.44594117
CTCGTTT121250.08.2797091
CTCCGAT109150.08.1861531
GTCGCTT64200.08.1142011
CCCGTAT37600.08.0741841
ACGTATA25500.07.98224218
GTCGTTT88350.07.9267171
TTCGATA48150.07.8813532
GTCCTAT76200.07.8776761
TATCGTA43900.07.85876845
CGAGACG56300.07.782171260
TCGTTTT135650.07.68053532
Produced by FastQC (version 0.11.2)