FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba120.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba120.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49024567
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT81300.011.2874761
CTCGTTT133850.09.1498861
TCGTATC66900.08.9212772
CGTATCT82150.08.399323
CCCGTCT117750.08.2621651
GTCGGTT110600.08.2348271
CGCTAGT19450.08.1591311
GTCCGCT80450.08.1476671
TATGCCG37300.08.13979948
CGGGTAT37300.08.139161
CCGTAAT76850.08.0804271
CTCCAAT339200.07.9432991
TCGTTTT174700.07.9385322
CCCGTAT47900.07.92251161
GTCACAT307800.07.85697031
CCCGTTT92100.07.79129031
GTCGGAT43950.07.7710911
CTCCTAT142850.07.70399761
CTCCATT403000.07.64455371
GTCGTTT124450.07.6233081
Produced by FastQC (version 0.11.2)