FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba119.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba119.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35604637
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT63700.011.7522651
CTCGTTT106200.011.1097151
CCCGAAT59650.010.6994781
GACGTAT22550.010.40326817
CGCCTAT16750.010.0921251
CCCGTAT34450.09.713681
CTCGAAT144700.09.655791
CGGCTAT30850.09.5052841
CCCGATT32000.09.4871121
GTCGGTT81750.09.4106241
ACGTATA24950.09.40271218
ACGATAC41100.09.40114160
CCCGTTT71400.09.2769321
TCGTTTT145550.09.2201912
CGAAATA31850.09.2076458
TCGTATC55250.09.1163952
AATCCGA55550.09.00551224
CGTAGTA48000.08.98433666
CGCTATT16250.08.9165581
TAACGCG14100.08.80829916
Produced by FastQC (version 0.11.2)