FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba118.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba118.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43749525
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT36700.015.98049817
AATCCGA89100.014.55908124
ATCCGAA88500.014.54075425
CGTATAG41350.014.26691419
ACGTATA41650.013.99847318
CGTAGTA87200.013.9263666
AAATCCG95650.013.27326523
GACTACG43850.012.98163135
AGACGTA48700.011.83041416
ACTACGT47750.011.63236236
TGTCGTA108750.011.54744863
GTTGTCG117300.010.73493361
ACGATAC67000.010.65864660
GTCGGTT99450.010.4761971
CCGCGTA15500.010.46147728
GCGGCTT122900.010.35823815
CTCGTAT74500.010.3264271
CATACGA71800.010.186275557
CCGTAAT67750.010.1331681
CTCGTTT131250.010.0933061
Produced by FastQC (version 0.11.2)