FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49774759
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT41300.014.20060717
ACGATAC71400.013.72233960
AATCCGA97650.013.42568324
CGTATAG46350.013.10010819
CGTAGTA96800.013.0798666
ACGTATA46600.013.02982818
ATCCGAA101250.012.88012425
CTCGTAT90600.012.5657911
GACTACG49400.012.43105735
CATACGA79800.012.32108957
CGATACT82650.012.23014261
CTCGTTT155850.012.1747481
AAATCCG104050.012.06907223
GCATACG83450.011.98889756
TCGAATG115800.011.6187692
CTCCGAT147000.011.4526651
ATACGAT87450.011.44050858
TACGATA89200.011.37077459
CTCGAAT211000.011.16715051
AGACGTA55150.011.13475816
Produced by FastQC (version 0.11.2)