FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba116.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba116.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55718112
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60950.030.05654743
CTCGTAT58550.029.75650842
TCTCGTA60550.028.88746341
CGTATGC64100.028.36435144
AATCTCG67350.026.1244639
ATGCCGT71400.025.56218347
TGCCGTC77550.023.66849748
GCCGTCT79400.023.42111449
TATGCCG84000.021.93235646
ATCTCGT80250.021.83909440
CCGTCTT84250.021.82697150
CGTCTTC96650.019.2049851
GTATGCC141050.013.45273645
CGTCTGA178300.011.00964516
ACGTCTG195600.010.30041815
GTAATCT183500.09.983314537
ACACGTC210300.09.66263313
GCTTGAA228750.08.85287559
CACGTCT233200.08.83212614
TGTAATC242200.07.905592436
Produced by FastQC (version 0.11.2)