FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba115.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba115.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37503190
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1123010.2994438606422547TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT172300.056.26547242
TCTCGTA172200.056.19774241
TCGTATG178950.055.080743
ATGCCGT180050.054.89945647
CGTATGC183350.054.4929544
TGCCGTC180950.054.35954348
GCCGTCT179450.054.21762549
CCGTCTT182800.053.18578350
CGTCTTC184650.052.26033451
ATCTCGT191150.050.9692740
CATCTCG193800.050.4325439
TATGCCG198700.050.3008146
GTATGCC219700.045.75953345
GATCATC253450.039.77434536
GCTTGAA259750.037.74670459
CGTCTGA291100.035.7670816
TGCTTGA277900.035.25644758
ACGTCTG299400.034.7755915
AGTCACC310700.033.2568128
ACACGTC315950.033.09707613
Produced by FastQC (version 0.11.2)