FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba114.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba114.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37066148
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1452850.3919614198918107TruSeq Adapter, Index 6 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA205500.061.09177441
CTCGTAT206900.060.7453442
TCGTATG209350.060.3312343
CGTATGC209750.060.01912344
CGCCAAT211750.059.5822633
ATGCCGT211900.059.3643547
TATCTCG215450.058.22236639
TGCCGTC216850.058.07320848
TATGCCG218550.057.6977746
GCCGTCT218400.057.56619349
CCGTCTT219100.057.3346550
ACGCCAA222550.056.93893432
ATCTCGT222200.056.4847440
GTCACGC225800.056.21175429
CGTCTTC228100.055.1931451
TCACGCC234500.054.0968630
CACGCCA242650.052.4072131
AGTCACG244650.051.9230828
CGTCTGA257700.049.8951216
GTATGCC260450.048.61379245
Produced by FastQC (version 0.11.2)