FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba113.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba113.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42920186
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7777141.812000535132816TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG690390.16085438213152198TruSeq Adapter, Index 5 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1130850.065.9492643
CTCGTAT1013850.065.92370642
CGTATGC1143600.065.8143344
TCTCGTA1008600.065.7774941
ATGCCGT1146250.065.5982547
TGCCGTC1156850.064.7648648
GATCTCG1024500.064.7462339
ATCTCGT1023550.064.7119540
TATGCCG1165850.064.7034446
GCCGTCT1149400.064.62318449
CCGTCTT1155650.064.3508650
CGTCTTC1157450.063.86894651
CGTCTGA1233800.062.95606216
GTATGCC1201350.062.88849345
ACACGTC1240050.062.6771913
ACGTCTG1243150.062.52681415
GATCGGA1270950.061.4842261
ATCGGAA1281100.061.481892
CACGTCT1263350.061.4771814
CACACGT1282150.060.67714712
Produced by FastQC (version 0.11.2)