FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38068277
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2902430.7624274668380709TruSeq Adapter, Index 4 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT392050.062.79559742
TCTCGTA391300.062.6245241
TCGTATG408100.062.540943
CGTATGC408800.062.44241344
ATGCCGT409900.062.21993647
AATCTCG395400.061.765539
TGCCGTC415850.061.255148
TATGCCG419100.061.0321646
GCCGTCT418500.060.85057449
CCGTCTT419850.060.52295350
ATCTCGT406700.060.10874640
CGTCTTC426100.059.61067651
CGTCTGA457400.056.73473416
ACGTCTG466050.055.7479815
ACACGTC467900.055.69145613
GTATGCC460050.055.56155845
CACGTCT486400.053.5020414
CACACGT499800.052.32473812
GCACACG502550.052.17578511
GATCGGA511900.051.4209181
Produced by FastQC (version 0.11.2)