FastQCFastQC Report
Tue 14 Nov 2017
HMKLYBGX3_n01_ba111.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLYBGX3_n01_ba111.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38935864
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6245971.6041688454634009TruSeq Adapter, Index 2 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG826800.065.6951343
CTCGTAT820850.065.6587342
TCTCGTA818700.065.5949241
CGTATGC832650.065.4988344
CGATGTA850200.065.4205434
ATGCCGT834850.065.1799747
TATCTCG828750.064.9992139
CACCGAT858900.064.8748631
ACCGATG859700.064.8105632
TATGCCG843850.064.7114346
CCGATGT860550.064.61825633
TGCCGTC841100.064.5482248
ATCTCGT834350.064.5423540
CCGTCTT838100.064.3052750
GCCGTCT838850.064.17827649
CGTCTTC838000.063.7774851
GTCACCG879450.063.38321329
TCACCGA879900.063.29982430
CGTCTGA902050.062.4206916
ACACGTC908650.062.0862713
Produced by FastQC (version 0.11.2)