FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26919802
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4835771.796361652288527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA16850.012.7181119
TCCGATC16800.08.2806841
CCCGGTT17550.08.2001481
CCGGTAT15353.6379788E-128.1253581
TAACACG27950.07.5450772
CGGTGAT21600.07.32888171
CGGTTAG13103.897003E-87.27682738
CCGGGAT33050.07.25731661
ACACGTG31100.07.22774554
AACACGT30700.07.17368273
GGGGAAT55200.07.12610631
GGGAAAT64350.07.00739861
CGGGTAT13859.6086296E-86.92720031
CGGGGTT41650.06.9105681
CCGGCAT22101.8189894E-126.7289451
GGAGAAT96000.06.6459631
CCGATCT23250.06.59659432
GTGCGCG32750.06.54914438
CGGGGAT36900.06.5001171
CGGCTAT17754.8803486E-96.4862011
Produced by FastQC (version 0.11.5)