FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48199720
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTT189650.09.322751
CGGGGAT152900.08.828051
CGCGGGT110000.08.0798431
GGGGAAT253000.07.98403361
CGGGAAT106100.07.8392881
CGCGAAT23850.07.7719531
GGGGGAT251050.07.7431391
GATCGGA461900.07.69668694-95
AGATCGG536400.07.491606794-95
ATCGGAA445100.07.34650894-95
GGGGTAT137550.07.187151
CTCGTAT48750.07.11708551
CGGGATT93400.07.07329651
CCGGGAT152450.07.0458761
CTCGGAT138400.07.03998471
GCGTCGT208800.06.988082494-95
GTCGGAT60950.06.94017271
CCCGGAT153450.06.9070391
CGCGGAT57300.06.8845791
CGGGTAT55050.06.8206181