FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48383748
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA55150.016.526489
CTACCGT71700.013.7048868
CGGGGAT154750.010.8109291
CGCGGGT91500.010.7003291
ACTACCG93550.010.1487017
CGGGTAT47700.09.6650551
CGGGGTT161350.08.7780551
GGGGAAT255600.08.4420131
CCGTATG64950.08.44159210-11
CGTATGG56950.08.29377912-13
GCGTCGT320150.08.26155194-95
CGGGATT82700.08.103351
CCGGGAT134450.07.95376441
GGGGGAT245450.07.9003831
GATCGGA759800.07.819168694-95
ACCGTAT61850.07.790186410-11
CGGGCAT194800.07.661111
AGATCGG852900.07.536835794-95
CGGGTGT145300.07.4906731
AGCGTCG374400.07.483380394-95
Produced by FastQC (version 0.11.5)