FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19027641
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA36150.019.6972269
TAACACG58450.014.9541772
AACACGT68800.013.1822393
ACGTGCG80250.013.13211256
CGCGGAT21850.012.181831
GTGCGCG63850.011.8954278
CTAACAC87750.011.4290811
CGCGAGT35900.010.97485810-11
ACACGTG84950.010.730374
CGCGTAT3850.00327788889.8765491
CGGGAAT38550.09.7404421
CGCGATT7351.9600884E-79.7001821
CGTGCGC101450.09.6391177
CACGTGC101250.09.3302625
TCAAACG58600.09.3158238
CGAGTCG44800.09.21851712-13
CGCGAAT8451.5276055E-78.9999321
CGGGGTT79950.08.9176161
CGACCTA9701.5275873E-88.8100364
CGGGCAT74600.08.7925791
Produced by FastQC (version 0.11.5)