FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22093710
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA40850.016.268499
TAACACG59850.012.5408132
AACACGT77200.010.7024163
ACGTGCG82950.010.7014686
GTGCGCG70750.010.131228
CGGGAAT43450.09.9545021
ACACGTG87550.09.435384
CTAACAC97300.09.3301471
CGCGAGT41000.09.14638710-11
CCGGTAT35250.09.0340741
CGGTATT38800.08.6927942
CGGAAAT44550.08.6418211
CGGCAAT38150.08.5965161
ACGGTTA23300.08.1492257
GCCGATT23450.08.1074661
GCGGTAT31700.08.0965961
CGATCCG22450.08.04501478-79
CGAGTCG48750.07.9844712-13
GATCGGA295700.07.91574694-95
CGGTTAG26550.07.86682378
Produced by FastQC (version 0.11.5)