FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20929122
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA35100.015.2821589
TAACACG56200.011.5799012
AACACGT68850.010.7585393
ACGTGCG81100.010.0674596
CGCGTAT4600.00135447489.2995911
CGCGAGT33950.08.80858910-11
ACACGTG82350.08.7625534
CCGGTAT31200.08.6835931
CCGGGAT89550.08.6516961
CGGGGTT94550.08.4455311
GTGCGCG68050.08.3009158
CGCGAAT8601.8913161E-68.2903341
CGGGAAT43900.08.2286441
CGCGGAT24450.08.1648771
GCGGTAT26450.08.0866011
TCGCACG40650.07.76532124-25
CGTGCGC93700.07.75100337
CTAACAC96850.07.70511671
GATCTAA54850.07.61595875
GGGGAAT131600.07.51253751
Produced by FastQC (version 0.11.5)