FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38668934
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA67800.015.3329169
TAACACG107850.010.923382
ACGTGCG138800.010.2257176
AACACGT131750.09.40632253
ACACGTG152750.08.5157724
GTGCGCG132900.08.4650668
CCGGTAT54800.08.4130041
CGCGAGT67250.08.29375910-11
CGGGAAT76250.08.2903281
CGATCCG41650.08.27323978-79
CCGGGAT165750.08.2584561
CGCCAAT47900.08.1365121
CGGGGTT169700.07.75814341
TCGACCG41400.07.739444726-27
GATCGGT80400.07.7345515
CGCGTAA22900.07.67937951
GATCGGA384350.07.61733594-95
ACGGTTA52450.07.60225537
CTAACAC180150.07.59832951
GTTACGA36300.07.58535774
Produced by FastQC (version 0.11.5)