FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61824632
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA99750.014.2287589
TAACACG171350.010.1746852
CCGGTAT100500.09.9798421
ACGTGCG221100.09.6183936
AACACGT212750.08.9051233
CGGTATT112350.08.8794332
GTGCGCG184600.08.8200298
CGGGGTT267900.08.6587331
CCGGGAT257750.08.3542381
CGGGAAT116350.08.2526271
CGCGAGT99150.08.01909810-11
CGATCCG61450.07.92777378-79
ACACGTG244000.07.76312734
GGGGAAT405750.07.72029971
TCGACCG66100.07.50451226-27
CGCCAAT75550.07.4872061
GTGTCGA77500.07.4120324
CTCGGAT189900.07.3341521
CGGGCAT236650.07.3114271
GATCGGA725500.07.28481894-95
Produced by FastQC (version 0.11.5)