FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58942460
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA94500.015.1197339
ACGTGCG198850.010.5274586
TAACACG179100.09.6546692
CGATCCG73400.09.61586678-79
CTACCGT72150.09.6056748
GTGCGCG163250.09.5665218
TCGACCG74250.09.33384826-27
CCGGTAT112550.09.0796641
AACACGT204500.08.9857323
CGGGGTT216400.08.7638011
GTTACGA53800.08.5593894
CGGGAAT116900.08.2538831
CGGGCAT209350.08.2188761
CGCGAGT97150.08.20860810-11
CCGGGAT226150.08.1337581
TACCGTA74350.08.1083459
CGGAGAT200900.07.92577361
GTTCGAC92550.07.872844724-25
CGGTATT119500.07.83122872
CGCGTAT12201.1012162E-87.7919651
Produced by FastQC (version 0.11.5)