FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51841476
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA91850.010.6466079
ACGATCG57600.09.8095873
GTTACGA40750.09.5531344
TCGACCG61950.09.38662326-27
CGATCCG61800.09.15180778-79
ACGTGCG163300.08.9824816
CGCGATT26350.08.47765351
CGGGGTT272600.08.2643931
CGCGGAT49050.08.2364181
CGGGAAT103650.08.2080911
GTTCGAC74250.08.11925924-25
CGAGAAT120550.08.0430611
AACACGT156750.07.84546143
GTGTCGA61550.07.7131464
CGATTCA92350.07.7110584
TTACGAC50600.07.69291835
CAACGAT140000.07.67253451
GCGTCGT330800.07.629013594-95
CTACCGT60700.07.5858778
CGACCGT60900.07.56083328-29
Produced by FastQC (version 0.11.5)