FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n02_ALm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n02_ALm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48136905
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA80050.014.5281749
ACGTGCG154000.011.0042536
GTGCGCG128400.09.7598868
CGCGAAT20550.09.7137571
CGATCCG62000.09.62082878-79
TAACACG146400.09.572032
TCGACCG63300.09.56120926-27
CGGGGTT203100.09.4775251
GTTACGA42750.09.4393174
AACACGT163100.09.2870533
TTACGAC49250.08.9638915
GTTCGAC73550.08.6158524-25
CGAGAAT103900.08.59987451
CGCGGAT45400.08.479671
TACCGTA57900.08.2803589
TGTTACG49450.08.2579723
CTACCGT58450.08.2024348
ACACGTG191300.08.1150374
AATCCGA76650.08.1129535
ACGATCG39900.08.0923983
Produced by FastQC (version 0.11.5)