FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26919802
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4604901.7105995058953258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC165000.025.3696528
TCGGAAG183600.023.6552053
ATCGGAA188700.023.4034672
GATCGGA185050.022.7898081
CGGAAGA197300.022.1807844
ACACGTC96200.021.36202812-13
ACGTCTG96750.021.0685314-15
CGTCTGA98600.021.03353516-17
CACGTCT100500.020.51823414-15
TCTCGTA55650.020.51815442-43
CTCGTAT47350.020.35815644-45
CACACGT106800.019.73008512-13
ATGCCGT60350.019.60418748-49
TGCGCGA14650.017.4741299
GCACACG122900.017.02996410-11
ATCTCGT62150.016.30537242-43
CGTATCT18900.015.82684238-39
TCGTATC18700.015.35626938-39
TATCTCG18350.015.21972140-41
AGCACAC157000.014.16146410-11
Produced by FastQC (version 0.11.5)