FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48199720
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60600.015.36212844-45
CGTATGC61250.015.08272746-47
TCGTATC70700.014.17785538-39
CTCGTAT69700.013.69718344-45
TATGCCG73950.013.03839748-49
TATCTCG75550.012.7945440-41
CGTATCT81100.012.41830938-39
ATTCGTA88300.012.10553336-37
TTCGTAT90250.011.84397336-37
AATTCGT101350.010.50008734-35
CACGAAT100700.010.47322930-31
ACGAATT106950.010.12794532-33
GTCACGA108700.010.07351928-29
TCTCGTA103000.09.47645642-43
CGAATTC121600.09.22031432-33
GAATTCG123150.09.02713834-35
CGGGAAT102200.08.6470351
AGTCACG129850.08.48761828-29
CCGGGAT150850.08.0945551
ATCTCGT123100.08.02558342-43
Produced by FastQC (version 0.11.5)