FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48383748
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA56200.017.4949869
TCGTATG63050.016.91206744-45
CGTATGC69700.015.9799246-47
TATGCCG72200.015.68970648-49
TCGTATC75450.015.61482338-39
CTCGTAT74600.015.05766944-45
CTACCGT75600.013.6337778
CGTATCT84950.013.30939238-39
TATCTCG95150.011.88115740-41
ATTCGTA106900.011.84376336-37
TTCGTAT111050.011.35837536-37
TCTCGTA102200.010.73570342-43
ACTACCG97550.010.2251327
ACGAATT128850.010.08438532-33
CACGAAT124550.010.01264130-31
AATTCGT123950.09.96564434-35
CCGTATG65350.09.70320510-11
ATCTCGT126300.09.40169442-43
GTCACGA131800.09.28125528-29
CGCGGGT97300.09.0338831
Produced by FastQC (version 0.11.5)