FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19027641
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT918520.48272930942937176TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC119900.040.3862146-47
TCGTATC128350.039.9543738-39
CTCGTAT120850.039.7348744-45
TCGTATG121450.039.47990844-45
TATCTCG124550.038.25105340-41
CGTATCT128250.038.05940238-39
AATTCGT146950.036.79090534-35
TATGCCG131450.036.7472548-49
GTCACGA148250.036.73826228-29
ACGAATT151450.036.62331432-33
GAATTCG153600.035.9403834-35
CGAATTC153350.035.5189732-33
TTCGTAT150850.035.33527436-37
AGTCACG157450.035.05925828-29
CACGAAT156250.034.96505430-31
ATTCGTA164300.033.2811836-37
TCTCGTA142800.033.22829442-43
ATGCCGT144200.033.08637248-49
GTATGCC149500.032.53297846-47
ATCGGAA338100.032.3997732
Produced by FastQC (version 0.11.5)