FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22093710
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1106640.500884640922688TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG139650.040.83090644-45
CGTATGC143100.040.5599146-47
CTCGTAT142700.040.04142444-45
TCGTATC155500.039.7151338-39
TATCTCG147950.039.00844640-41
CGTATCT153000.038.51663638-39
TATGCCG154450.037.65622348-49
AATTCGT172550.037.4998734-35
ACGAATT179400.036.74321732-33
GTCACGA177300.036.7203228-29
GAATTCG181700.036.10824234-35
TTCGTAT179150.035.56085636-37
CGAATTC184600.035.00046532-33
CACGAAT187800.034.64338330-31
AGTCACG190900.034.50246428-29
TCTCGTA166600.034.0410242-43
ATTCGTA194750.033.44415736-37
ATGCCGT171500.033.400248-49
GTATGCC177450.032.90924546-47
GAGCACA420700.031.7147549
Produced by FastQC (version 0.11.5)