FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20929122
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT683530.32659277345700405TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC94000.038.80601546-47
TCGTATG94350.037.4540544-45
CTCGTAT93200.036.97338544-45
TATGCCG106000.034.41271248-49
ATGCCGT119450.029.46426848-49
TCTCGTA117750.028.61979342-43
ATCTCGT127600.027.76918842-43
GTATGCC130350.027.63822746-47
ATCGGAA289600.027.0998522
GATCGGA292050.026.7143751
TCGGAAG297100.026.5595253
GCCGTCT133150.025.09499250-51
TGCCGTC142900.025.06127750-51
GAGCACA324150.024.9835939
AGAGCAC327000.024.6204768
CGGAAGA323150.024.4919994
AATCTCG145400.023.63577840-41
CGTCTGA174300.023.4078216-17
CCGTCTT152800.022.90927552-53
ACACGTC178800.022.14183412-13
Produced by FastQC (version 0.11.5)