FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38668934
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1168410.30215728212212933TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC164750.038.0117446-47
TCGTATG164450.037.3447644-45
CTCGTAT165300.036.03211244-45
TATGCCG187200.033.71943348-49
ATGCCGT214000.028.84188348-49
TCTCGTA207400.028.35192542-43
GTATGCC229300.027.33181246-47
ATCGGAA526050.026.3234862
ATCTCGT232600.026.31148542-43
GATCGGA528700.026.0342921
TCGGAAG535900.025.9636863
GCCGTCT231800.025.02897850-51
TGCCGTC248750.024.63143550-51
GAGCACA592550.024.040229
CGGAAGA583900.023.8702454
AGAGCAC595850.023.7714068
CGTCTGA308200.023.33998716-17
AATCTCG263550.022.63717840-41
ACACGTC318850.021.97276512-13
CCGTCTT277250.021.79111952-53
Produced by FastQC (version 0.11.5)