FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61824632
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1908570.308707053848699TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC276250.038.0746646-47
TCGTATG271200.037.6716344-45
CTCGTAT272200.036.43393344-45
TATGCCG315800.033.28359248-49
ATGCCGT351450.029.11681448-49
TCTCGTA344700.028.4473342-43
GTATGCC375950.027.76268246-47
ATCTCGT382700.026.48523742-43
GCCGTCT380400.025.49621850-51
TGCCGTC415600.024.66817550-51
ATCGGAA949800.023.7973442
TCGGAAG954850.023.7410963
GATCGGA953300.023.5645581
GAGCACA1000800.023.227259
AGAGCAC1006100.022.8828878
CGTCTGA525450.022.35861616-17
CCGTCTT453800.022.25154552-53
CGGAAGA1032200.022.0771394
AATCTCG452850.021.84370640-41
GTCACAT567250.020.38797828-29
Produced by FastQC (version 0.11.5)