FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58942460
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1374990.23327665659017288TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG209900.035.66232344-45
CGTATGC219400.035.4927146-47
CTCGTAT208550.034.94801744-45
TATGCCG232550.033.55710648-49
GATCGGA566700.029.0517121
ATCGGAA574250.028.7472972
TCGGAAG608500.027.3711763
TCTCGTA277000.026.0206942-43
ATCTCGT298600.025.13258642-43
ATGCCGT303900.024.91270448-49
GTATGCC321150.024.15881246-47
CGGAAGA699800.023.854564
GAGCACA742250.022.9989729
GCCGTCT311700.022.90267450-51
ACACGTC367550.022.74457212-13
AGAGCAC765650.022.165728
TGCCGTC353150.021.57965750-51
CACGTCT401700.021.50238814-15
CGTCTGA404150.021.41874916-17
ACGTCTG393950.021.17787214-15
Produced by FastQC (version 0.11.5)