FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51841476
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2509300.48403328639794135TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC337500.040.88258746-47
TCGTATG330800.040.43287744-45
CTCGTAT324250.040.05579844-45
TATGCCG365950.037.78853248-49
ATGCCGT395950.033.8697848-49
TCTCGTA378750.033.82819442-43
ATCTCGT403250.032.64453542-43
GTATGCC425600.032.17987446-47
GCCGTCT407700.031.11686350-51
TGCCGTC442600.030.36413650-51
GAGCACA1031900.029.4635479
TCGGAAG1046550.028.6603163
AGAGCAC1076850.028.079278
ATCGGAA1065700.028.0517792
CGGAAGA1077500.027.8371874
GATCGGA1076250.027.6354851
CCGTCTT492450.026.81305952-53
AATCTCG484200.026.71730640-41
GTCACAT593750.025.4503728-29
CGTCTTC510100.024.5305152-53
Produced by FastQC (version 0.11.5)