FastQCFastQC Report
Wed 17 Oct 2018
HMKLWBGX7_n01_ALm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKLWBGX7_n01_ALm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48136905
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1415040.2939615664945638TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG202650.038.08664344-45
CGTATGC208900.038.049846-47
CTCGTAT204450.036.32258244-45
TATGCCG230600.034.5411748-49
ATGCCGT274700.028.17467948-49
TCTCGTA262700.028.02490442-43
GTATGCC296150.026.95205346-47
ATCTCGT283100.026.91983442-43
GCCGTCT285700.025.80974850-51
TGCCGTC315900.024.5301350-51
GAGCACA808350.021.7998079
CCGTCTT361750.020.98778752-53
AATCTCG356100.020.96903240-41
TCGGAAG827000.020.805753
AGAGCAC857000.020.5069188
ATCGGAA847300.020.2848282
GTCACAT437800.020.09048528-29
GATCGGA853950.020.0358731
CGGAAGA874600.019.765864
ACACGTC447800.019.3415612-13
Produced by FastQC (version 0.11.5)