FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89248529
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65071447.291037816432806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT21600.024.166862
TCCGATC24100.021.6603741
TCCGATT15600.015.8017361
CCGACGC12250.014.201752145
CGATCTA15100.013.9268813
GACGCGC11501.4551915E-1111.976295145
CGACCCG6253.6536898E-511.598098145
CCGATTT21950.09.9089632
ATGAGCG25850.09.2538145
GCACGCG11854.3921864E-79.17571145
TACCCGC8004.431751E-49.061014145
GTTCCGC14901.6940248E-88.756952145
CGGTTCG12600.07.83157410-14
CCGTATA19151.8788342E-87.57056145
CGATCTG22851.1859811E-97.2991863
CGACGCT13953.769908E-57.28188949
AGTACGG12003.983382E-47.2502091
GCCACGC17001.2871024E-67.248811145
ACGAGCC14506.356025E-56.998852145
CGGAGTC54250.06.5506724
Produced by FastQC (version 0.11.5)