FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_umtu075_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_umtu075_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8319029
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2186082.6278066827270345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG60050.09.6591121
TAATAAT460950.08.1469363
TAATAAG135150.07.8867496
ATAATAG160150.07.65167476
ATAATAA612300.06.54742
AATAATA672250.06.4387821
CGCCGTA37150.06.24561145
TAGGGGG43251.8189894E-125.8673521
ATTATTA390450.05.60791871
CGGAGGG15650.0079089465.5596905145
TCGCCGT37750.05.530172140-144
TGGTCGC44157.094059E-115.419596145
AGGGGGG194400.05.4079411
GCCGTAT43450.05.2050858140-144
TATAAGG86800.05.0961957
ATTGGGG52751.4551915E-115.0855681
Produced by FastQC (version 0.11.5)