FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_umtu067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_umtu067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809555
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1786272.6231816910209256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG13300.021.8103528
GAGAGCG14450.016.560927
GAGCGGG17450.012.052479
TGGTCGC21903.8871804E-97.2833967145
GGAGAGC46900.06.33871946
GGGCCGA10300.06.33509455-59
GTCGCCG17701.2642931E-46.1443143145
TCGCCGT15550.00111657876.0613365145
ATGGGGG66850.05.965131
CCGAAAA15700.05.818431460-64
GGGGAGA97600.05.64522084
GGCCGAA9301.8189894E-125.613029555-59
CGTGTAG73200.05.5466704145
GGGAGAG111500.05.52700575
GGCCCGA8401.03682396E-105.52393455-59
GCCGAAA8806.548362E-115.437462360-64
CCGATAA6203.4902969E-65.145126360-64
Produced by FastQC (version 0.11.5)