FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_umtu041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_umtu041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6990776
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1323921.893809785923623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC450.00963275848.3444757
TTTCGGG10609.828122E-68.8917141
TAATAAT387750.07.2548421
ATAATAG148000.06.8590516
ATATAAG132050.06.8089646
TATATAA438800.06.24576475
ATAATAA461000.06.14887863
TAATATA339450.05.98041
AATAATA516150.05.81476352
CGGTGGT26305.745307E-65.5135927145
ATGGGGG55400.05.3656471
TCGGTGG27109.7106295E-65.35083145
CGCCGTA12550.05.1994715145
ATATATA969900.05.0527452