FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_sian047_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_sian047_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233653
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1132342.67461693246943No Hit
AATAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTCC120450.28450607548611095No Hit
TATTATATATTAGGGCCCGTCCCTCAAACTATGTCACTGGGTAGATCTCA72720.17176655715525102No Hit
TAATAATAATAATTGAATTTCAGATAAACAATGAATAGTTTTTAGTATTT52110.12308519380308212No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47250.1116057456763698No Hit
TATTAGGGCCCGTCCCTCAAACTATGTCACTGGGTAGATCTCAATCCTAG45660.1078501237583713No Hit
TAATAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTC45330.10707065505840938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTCCG1100.0096738726.364296145
TGAAACG3103.1060798E-416.371376145
GTTGTCG3153.5087674E-416.111513145
TGGAACG4250.003369467311.941475145
CTGATCG2551.0004442E-1011.3756557
TCGCCTA2803.6379788E-1210.87334525-29
TAATAGC8154.2361444E-610.6777637
TATCCCC8054.3075823E-59.9096568
GGCACTG11803.9170118E-89.830753145
ATCCCCT9101.5727304E-59.5636169
AGACGGT6100.00448390059.5087721
CAATAGT19100.09.4893184
TATTGGG7652.823209E-49.4776961
GTCTAGT3851.6318336E-79.4181577
CGTTCTG2652.3492248E-89.30293315-19
CTCGTTC2857.861672E-99.15892415-19
CGCCTAG3452.8921932E-108.82474425-29
GGTTAAT6950.08.76258230-34
TTTCTGA21650.08.7072231
ATTAGGG264450.08.6935659
Produced by FastQC (version 0.11.5)