FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_sian034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_sian034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276789
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312502.4475461489721484No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA115070.9012452331591203No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT85290.6680038753466704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGCG2558.913048E-1131.2806957
AGAGCGG2752.1282176E-1029.0068728
GAGCGGG3102.4121618E-823.3935579
TAATAAT46450.013.894031
CGCACTA750.001317205413.531768105-109
GGAGAGC6857.2282674E-611.6437266
GGGCCGA1552.3220764E-611.22714755-59
GTGGGGA14901.6370905E-1110.216942
CCGAAAA2601.7398452E-78.92351360-64
AATAATA63700.08.8765252
GAGCGTC23800.08.834743145
GGTCGCC6650.0091048338.722504145
GCCGAAA1902.624164E-48.39514760-64
ATGGGGG13001.6519334E-68.3670391
GGGGAGA17855.0458766E-98.1229464
ATAATAA56450.08.0902963
TAATATA44850.08.0840961
GGCCGAA1800.00142372188.05651855-59
GCGGTAT1650.00587491557.9079905100-104
ATAATAG18901.4562829E-87.6728746
Produced by FastQC (version 0.11.5)