FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_sian020_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_sian020_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8423809
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1073911.274850842415824No Hit
AATAATAATAAGAAAATCATCTCTGCCTAAAAATTTAAGTTAAACTCCAT113920.1352357348083272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCATCG1053.8260114E-641.430935145
CGAGACA5600.033.662636145
ATGAGCG16450.022.919243145
CGTCGTT2953.8793223E-722.119907145
TTCTGAG29900.019.1553611
ATAACAC40950.018.771658
ATTCAGG45350.018.54451
CCTGCCG3652.988707E-617.877733145
TCCGTCA4155.250931E-717.470879145
CGGAAGC3453.6821708E-516.812555145
TAATAAG206750.015.6059496
GATCGGG2800.002972731815.536601145
GCGATTG2850.003344703715.26403145
TTTCTGA38600.015.02578451
CACTCCC4902.9706862E-614.796763145
AATAAGA187700.014.795987
TCCGCCA3951.1702591E-414.684383145
CGCGTGC6602.158231E-814.281119145
CATCAGG5851.2380406E-613.633258145
TCCTGTA10151.8189894E-1213.572205145
Produced by FastQC (version 0.11.5)