FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_sian020_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_sian020_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11857717
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1823071.5374544695239396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT25200.09.782293145
GTCGCCG27450.09.244597145
GTGGTCG40600.07.1432567145
TAATAAT624000.07.0993551
ATATAAG238250.06.45249276
TAATAAG210500.06.40745266
CGCCGTA23000.06.366061140-144
GGTCGCC30350.06.352874140-144
CCGTATC28506.992159E-75.5968046145
TGGTCGC34250.05.5871534140-144
ATAATAG258150.05.56182966
ATAATAA777800.05.5175992
GCCGTAT27700.05.4966974145
AATAATA850800.05.47103931
TAATATA640850.05.30614041
GTCGAAG67150.05.2906904145
Produced by FastQC (version 0.11.5)