FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_sian007_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_sian007_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences387435
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105152.714003639320144No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTACAAGGGTGTAGATC28190.7276059209931989Illumina Single End PCR Primer 1 (96% over 33bp)
TATATATATATATATATATATATATATATATATATATATATATATATATA16050.41426303767083505No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT13100.33812123323912396No Hit
CTCAGAATGATTATTTTTAAGACATTCTTTGGATATATAAGAATATTTAT4120.10634041839276266No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT15350.034.9549679
AAGAGCG16450.032.6175548
CGGAAGA16600.032.3186455
TCGGAAG17250.031.0968254
GAAGAGC17450.030.748357
ATCGGAA17700.030.3101433
GATCGGA17900.029.967612
AGATCGG18700.029.0807251
GGAAGAG20600.026.0431796
AACGCAA500.001395663617.396965140-144
CGCCGTA6200.017.07092555-59
CCGTATC6200.016.8383860-64
CGTATCA6500.016.5073760-64
TCGCCGT6400.016.31091755-59
GTCGCCG6650.015.69772555-59
GCCGTAT6400.015.63250760-64
GGTCGCC7050.014.6014255-59
TAATAAT21700.014.0337951
TCTCGGT7800.013.756494545-49
GTATCAT7950.013.49659160-64
Produced by FastQC (version 0.11.5)