FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_krian027_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_krian027_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896110
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG136011.5177824151052886No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA126081.4069701264353707No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT93681.0454073718628294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGG1907.1528248E-626.7112568
GAGAGCG1751.25535E-424.8578227
CCGAAAA2159.209543E-910.7906360-64
GGCCGAA1759.915695E-69.94279755-59
GGGCCGA1502.3153736E-49.66660755-59
TCGCAGT1502.3156335E-49.66649990-94
TAATAAT35950.08.6724871
TAATATA29350.08.6463751
GGTCGCA3206.206392E-98.60922590-94
GCCGAAA1600.0044491958.15619960-64
AATAATA47200.07.0650822
CGAAAAA3758.051393E-76.95995760-64
TCGCAAT2450.00392237266.5100183100-104
ATAATAA43005.456968E-125.73207473
CGTATCA3150.0084937745.5236526135-139
GAAGAGC19450.00273828265.219526145
Produced by FastQC (version 0.11.5)