FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_khyl049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_khyl049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4643984
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG737431.5879253675292593No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATA60040.12928554448077342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGT7600.0027960618.586712145
TGGTCGC12250.0039156216.511103145
AGGCGTA20403.2182033E-66.397942145
CAGATCG12650.00547484966.305218145
CGCCGTA6452.3787652E-75.3959427130-134
Produced by FastQC (version 0.11.5)