FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_ISO_525_Jun28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_ISO_525_Jun28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5122252
Sequences flagged as poor quality0
Sequence length151
%GC27

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG986721.926340211297687No Hit
TATTATATATGTTTTATATTCTGTTATATATAACTATTTGAAATTATATA243510.47539636862848605No Hit
TAATATATATCATAAAAATGATATTTTATGTAAATTATGTTATGAAAGTA91130.1779100286358422No Hit
TAATATATATGTTTTATATTCTGTTATATATAACTATTTGAAATTATATA73200.14290589373580215No Hit
TAATAATAATATTAGTAACAACACATCTCTTTACCAGCAACACTTGAGCA71200.13900136112006983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGAA2450.038.47272145
CCCCGCG850.0027418734.120605145
CCGGTTG1100.00967114826.365921145
CACCGGC1409.995175E-425.895102145
TGGGACG2353.7105434E-521.597616145
ACCGGAC1750.003658923320.71608145
AGGGGAC1900.00588818119.0806145
ACAGCCG2307.9634215E-418.914684145
AGCCGAA2701.07955595E-418.797926145
GCCACAC4109.032434E-615.916013145
CAACCGG6158.5019565E-915.32653145
GCGACAA2906.358207E-515.0013145
CTCGCTT7050.014.4017878
CTGATCG9500.012.9745551
CCTGCCG4050.002346865412.53195145
TATATCA176650.011.9044936
TCGCTTT8700.011.6716679
ATATATC193150.011.4492375
ACAAGCG3900.002848224711.154813145
TGAGCCC10901.14159775E-810.643125145
Produced by FastQC (version 0.11.5)