FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n02_ISO_349_Jun28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n02_ISO_349_Jun28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13037196
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3204642.4580745737043457No Hit
TATATATATTTATGTATCTGAACTCTGTTCAGATATATGATTTTATATAT262420.20128561386973087No Hit
TATATATATAAACATAAATATTTTTATATATAAGATATATAATGTCTATT193350.14830643030909407No Hit
TAATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCACA189690.14549907817601268No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG172310.13216799072438581No Hit
TGCCCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAAC171950.1318918577276893No Hit
TGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTC163510.12541807302735958No Hit
AGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCAC160540.1231399758046132No Hit
CCAGGCTGAAATGCAGTGGCACAATCACAGATCACTGCAGCCTCAACCCC155450.11923576204576505No Hit
AAAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATC154100.11820026330815307No Hit
AACATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGG149660.11479462301556254No Hit
TGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATC149460.1146412157951756No Hit
TTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGA148160.11364406886266035No Hit
TTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAA147430.11308413250824795No Hit
TAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGAT146440.11232476676733248No Hit
CAACATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAG144740.11102080539404331No Hit
AAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCA144490.11082904636855963No Hit
GAGATCCTGTCTCAAAATATAATAATAATAATGTTGCCCAGGCTGAAATG141380.10844356409154239No Hit
CATTATTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCT140730.10794499062528476No Hit
TTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACA139810.10723931741150475No Hit
AGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGAT139190.10676375502830518No Hit
ATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAGA138800.1064646109485506No Hit
TTATTATTATATTTTGAGACAGGATCTCGCTCTGTTGCCCAGGCTGAAAT137050.1051222977701647No Hit
CAAAATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAAT134600.10324305932042442No Hit
GAGGCTGCAGTGATCTGTGATTGTGCCACTGCATTTCAGCCTGGGCAACA133550.10243767141339288No Hit
TGAGGCTGCAGTGATCTGTGATTGTGCCACTGCATTTCAGCCTGGGCAAC133220.1021845494997544No Hit
GACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACA132070.10130245798252936No Hit
AATATAATAATAATAATGTTGCCCAGGCTGAAATGCAGTGGCACAATCAC130700.10025161852287869No Hit
ACAGGATCTCGCTCTGTTGCCCAGGCTGAAATGCAGTGGCACAATCACAG130470.10007520021943368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGTCG5650.060.31068145
TGACACG657.3392753E-444.61609145
AGTCCCC1157.1485465E-637.826687145
AGCTACA7100.031.655428145
TCTCGCG1855.8224687E-627.432863145
CCAGCCG9500.025.947777145
AGCTACG1952.616807E-422.308044145
GTAGGAA4659.094947E-1221.8255392
CGAGACG3355.5108103E-821.642132145
TCTCGCA6450.021.356926145
AGGATCG7100.020.422855145
AATCCCC2205.907967E-419.773039145
CGAGTCG3051.2766961E-519.016693145
GTGATCG7650.018.954548145
TCGCGCG2307.968123E-418.913343145
CAGGACG1950.006844248618.590036145
TGTGCCC8250.018.454836145
CCTGCCG4506.3097104E-817.7225145
CGGCTAC2050.00912626217.68496
CTGCACG2050.00913130417.683205145
Produced by FastQC (version 0.11.5)