FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89248529
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10216871.1447662067349031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG36500.090.131037
GTCACTA137800.041.0163238
TCAGTCA194550.028.530855
TCACTAT208550.027.7619619
AGTCACT217450.026.82557
CCGACGC11500.023.317993145
GTCAGTC106550.022.6484584
ACTATCG30100.015.45555810-14
TAGGGGG68150.014.6744244
CGTACTA11500.014.4936514
CTATCGG14350.014.34110510-14
CAGTCAC421800.013.5030916
AGTACGG11800.012.90162851
GATCCGT11159.094947E-1212.347985
GTACTAG54500.011.8334315
TTTCGGG15350.011.3311912
CTATCCG8600.010.61666910-14
ACTAGGT68300.010.0789267
TAGGTTC71700.09.9042249
CCGCAGT19400.09.71153310-14
Produced by FastQC (version 0.11.5)