FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_umtu067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_umtu067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809555
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG111450.011.0578133
CGGAAGA112400.010.964034
GATCGGA112800.010.8652731
ATCGGAA114200.010.7920892
AGAGCAC139450.08.9399218
GAGCACA140150.08.895279
GAAGAGC153850.08.4330086
TATGCCG33800.07.635477545-49
GCCGTCT34800.07.6252850-54
TGCCGTC35300.07.352961550-54
ATGCCGT37100.07.30803545-49
CGTCTTC38250.07.08914950-54
CGTATGC36950.06.984550545-49
AAGAGCA190850.06.8360337
GGAAGAG195850.06.69856835
CTCGTAT39200.06.69376740-44
CCGTCTT39050.06.64685150-54
TCTCGTA40000.06.487406340-44
TCGTATG39500.06.423531545-49
TATCTCG42700.06.17904840-44
Produced by FastQC (version 0.11.5)