FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_sian047_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_sian047_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233653
Sequences flagged as poor quality0
Sequence length151
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTCC132380.3126850500029171No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77880.1839546131910197No Hit
TATTATATATTAGGGCCCGTCCCTCAAACTATGTCACTGGGTAGATCTCA57650.13617081985698873No Hit
TAATAATAATAATTGAATTTCAGATAAACAATGAATAGTTTTTAGTATTT56090.1324860587298959No Hit
TAATAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTC52240.12339225723033986No Hit
AAAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTCCA50180.11852648292148649No Hit
TATTAGGGCCCGTCCCTCAAACTATGTCACTGGGTAGATCTCAATCCTAG49890.11784149527606538No Hit
TAAAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTCC46200.10912561799467269No Hit
AAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTCCAT45630.10777926296746568No Hit
ATAAAATAATAAAATATGATGAAAACAAAAATGTCCCTAACAAGGAGTTC42830.10116558914960673No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAC1102.4399409E-432.948868145
GGCGCTT1100.0096831826.359095145
GGCGGAC2552.7145798E-622.74118145
GCGCTAA1750.00991312612.426431145
GGCGGCC6102.8489052E-511.883199145
CTCGTTC2457.016388E-811.8341375
GTTGAGC10254.3837645E-911.3151245145
CGAATAT5300.001398981910.94151145
GCGACAC4800.00835497210.571095145
CGTTCTG2151.17794025E-510.1138197
GGCCCGA13854.6020432E-109.944135145
ATAATAG55850.09.0848466
TTTCTGA25300.08.8861721
ACCCGCG4151.8063458E-48.733435145
TTCTGAG27500.08.7027151
ACAAGGG7500.0024867268.698501145
TAATAGG10105.4075565E-58.6117677
TTACAAG25900.08.395845
TAGTTAC26150.08.3168512
ACAAGTT27400.08.2005617
Produced by FastQC (version 0.11.5)