FastQCFastQC Report
Mon 1 Jul 2019
HMKHTBGXB_n01_sian034_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKHTBGXB_n01_sian034_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1276789
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATATATATATATATATATATATATATATATATATATATATATATATATA110390.8645907820321134No Hit
ATATATATATATATATATATATATATATATATATATATATATATATATAT83790.6562556538316042No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCATGATATCTCGTAT23150.18131421871585673TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATAAT53300.013.4680521
CGGAAGA31450.011.9851484
TCGGAAG31850.011.8346273
ATCGGAA32650.011.7675872
GATCGGA32900.011.6809121
GAGCACA35450.010.8368569
AGAGCAC36900.010.4110178
ATAATAG21350.010.185546
AATAATA65950.010.1127152
CCGACTG1150.002757195410.08476635-39
GAAGAGC36700.010.0731386
ATAATAA59550.09.4945313
AAGAGCA43900.09.0811717
GGAAGAG46300.08.2976595
TATGCCG9600.08.15537445-49
ATGCCGT10400.08.085669545-49
TAATATA49500.08.0566461
CCGTCTT10150.07.85771450-54
GCCGTCT9800.07.842406750-54
AGCGCAC1850.00186930917.836995645-49
Produced by FastQC (version 0.11.5)